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SPR NAVI™ 210A

SPR Navi™ 210A is based on the MP-SPR (Multi-Parametric Surface Plasmon Resonance) technology. It offers same unique features as SPR Navi™ 200, but is fully automated. The instrument is equipped with a built-in degasser, automated valves and syringe pumps that automate up to 6 samples. See the comparison with our other MP-SPR instruments. Also here, to fully enjoy the MP-SPR, we recommend the additional set of lasers.

Product Category: SPR

Industry: Life Sciences, Particle and surface science

Supplier: Bionavis

Description

SPR NAVI™ 210A

SPR Navi™ 210A is based on the MP-SPR (Multi-Parametric Surface Plasmon Resonance) technology. It offers same unique features as SPR Navi™ 200, but is fully automated. The instrument is equipped with a built-in degasser, automated valves and syringe pumps that automate up to 6 samples. See the comparison with our other MP-SPR instruments. Also here, to fully enjoy the MP-SPR, we recommend the additional set of lasers.

Key Features of SPR Navi™ 210A:

True Goniometric SPR

Unlike most SPR systems, SPR Navi™ 210A is a true goniometric SPR. This eanbles a wide angular scan range (= 40–78°) that produces a complete SPR curve with absolute angle information. The true goniometric SPR configuration confers great advantages as it widens the range of refractive indexes at which you can measure, allowing you to characterize surfaces in gas and/or liquid. It also allows you to check the quality of user-defined surfaces before sample injection, providing you with additional evidences. In other words, the wide angular scan range makes SPR Navi instruments sensitive and versatile.

Customizable flow cell

SPR Navi™ 210A comes with dual measurement channels for in-line referencing or duplicate measurements. In addition, the easily accessible flow-cell can be customized for user-specific experiments, thus broadening your experimental capabilities. For instance, BioNavis can provideelectrochemical flow cells or flow cells equipped with optical fibers for surface illumination. The flow cell shape can also be customized to tailor the flow dynamics to your needs.

Flexible sensor-slide system

With the SPR Navi™ 210A instrument, you can use the innovative sensor-slide holder for easy “drop-in” placement of your sensor-slide into the instrument. It allows users to change surfaces within seconds, making SPR Navi™ 220A an extremely convenient instrument to work with. The sensor-slide holders can be labeled and used for long-term storage of your custom-modified surfaces. Sensor-slidescan be modified outside the instrument, with a great number and combination of layers, prior to the actual analysis. This unique removable sensor system provides additional flexibility. It allows you to combine SPR data with other widely used techniques to provide supporting information, including surface topography, surface wettability, chemical composition, photochemical processes, swelling, adsorption...

Contamination free MP-SPR

The proprietary optical elastomer in the SPR Navi™ 210A finally does away with the need for messy and potentially contaminating index-matching oil. The result is a contamination-free environment for your measurements. The optional immobilizer, a horizontally oriented stand-alone flow cell, can be used for in-situ functionalization of SPR slides. This further minimizes the risk of cross-contamination and enables minimal sample depositions.

Specifications

SPR Navi™ FLOW-CELLS

Flow-cell type Inlet diameter Number of fluidic channels Number of optical channels Material Volume of one channel
SPR301 
Standard flow-cell for SPR Navi 220A
medium 2 2 polydimethylsiloxane (PDMS) 1 µL
SPR302-LS
Flow-cell for fast kinetic studies
small 2 2  polyetheretherketone
(PEEK)
1 µL
SPR305-MS
Standard flow-cell for SPR Navi 210A,
optimal flow-cell for material science measurements
large 2 2 PEEK 1 µL
SPR306-TI
High chemical resistance
large 2 2 Titanium and Kalrez material 1 µL
SPR310-GS
Gas flow-cell
2 2 PDMS
(Titanium optional)
1 µL or 5 µL (depending on application)
SPR321-EC
Electrochemical flow-cell
1 2 PEEK, NBR 70/ Kalrez 100 µL
SPR330-HR
High Chemical Resistance flow-through
cuvette
1 2 PEEK, Kalrez 100 µL
SPR305-Cust
Custom made flow-cell
custom diameter 1 / 2 2 PEEK, Kalrez 1 µL / 100 µL
SPR323-Cust
Custom made flow-cell with additional
optical access e.g. fluorescence

Specifications are subject to change without prior notice.

All flow-cells can be easily exchanged by the user. All flow-cells enable measurements in air and in liquid, but only gas flow-cell enables measurements with pressurized gas.

 


SPR NAVI™ SPECIFICATIONS

ANALYTICAL
PERFORMANCE
Measured property SPR Navi™ instruments measure light intensity reflected from the sensor surface (absolute measurement), while traditional SPR instruments measure light shift (only a relative measurement).
Refractive index (RI) range 1.00 - 1.40 (Can be extended using additional wavelength option)
Using the same optics (prism and sensor slides) and in the same automated scan.
Dynamic range 38 000 mdeg
Noise level 0.3 µRIU (short term)
1 µRIU corresponds to 1 pg/mm² surface coverage of a typical protein.
100-fold improvement using Selectively Amplified Surface Plasmon Resonance.
Working range kon = 10E3 < ka < 10E8 M-1 s-1
koff = 0.1 < kd < 10E-7 s-1
KD = 10E-3 - 10E-12 M-1
Fastest sampling rate from 4 ms
in Fixed Angle advanced timing mode
Temperature control 15-45oC  (ambient +20/-7oC)

Note on working range: Values ka and kd are also dependent of each other, as well as the Bmax (binding maximum) values of the assay at the time, so in principle it is completely sample dependent range and the given ranges are just a guideline.

Extending working range: The range can be extended with careful experimental design. Please refer to J. Mol. Recognit. 2005; 18: 307–317 for more information.
Another option to extend the working range is Selectively Amplified Surface Plasmon Resonance (SAMP-SPR) technology that can be applied with SPR Navi™ instruments having additional wavelength (785 nm). SAMP-SPR is ideally suited to measure low concentrations of analyte in complex biological mixtures, with 100 times increased sensitivity and significantly reduced interference from background non-specific binding. This technology can be used to extend the range of affinity.

INSTRUMENT
Technique Multi-Parametric Surface Plasmon Resonance (MP-SPR)
Measuring principle True goniometric SPR with rotating laser (true angluar resolution 0.001o)
Wavelength of light 670 nm (and 785 nm with additional wavelength option)
(other wavelengths available by request)
Optical channels 2
SPR angle scaning range 40o - 78o
(with the same optics and same sensor slides and in the same scan, no need for prism exchange!, no manual manipulation needed)
Prism coating Optical gel index matching
(no need for messy oil operation between measurements)
Sensor slides  Au, Pt, Al, TiO2, SiO2, CMD, ...
12 x 20 mm
Refractive index (RI) range for the running buffer/bulk 1.00 - 1.45
Dynamic range 38 000 mdeg
Temperature of measurement chamber 15-45oC  (ambient +20/-7oC) (in all models)
Temperature of sample storage 4-40°C at 25°C ambient temperature (only in 220A model!)
Computer requirements The minimum PC requirements are:
MS Windows® XP / VISTA / 7 (not Starter editions used in netbooks)
both 32- and 64-bit versions are supported
1xUSB 2.0
2 GB RAM
2 Gb HD
Power requirements 100W, 100-240V, 50/60Hz

LIQUID HANDLING SPR NAVI™ 220A

Liquid handling Integrated Autosampler The instrument is equipped with two autosampling areas, one for small volumes and another for large volumes:

  • Autosampling area 1:
    • Fully configurable program
    • Sample capacity: 1 microtiter plate (96 wells, both high and low format), 48 vials (1.5 mL, optional), or 12 vials (10 mL, optional)
    • Injection modes: Full loop, partial loopfill
    • Loop volume: 12 - 2000 μL programmable, exchangable, others by request
    • Temperature controlled sample storage: 4-40°C
  • Autosampling area 2:
    • Fully configurable program
    • Sample capacity: 6 samples in vials or bottles
    • Injection modes: Full loop
    • Wash: Programmable, wash between injections and wash between vials
    • Loop volume: 12 - 2000 μL programmable, exchangable, others by request. Loops are standard liquid chromatography loops and can be exchanged easily.
Integrated Syringe pump

  • Sample syringe: 2 500 µL, 1-channel
  • Measurement flow (running buffer)
  • 2-channel
  • 2500 μL standard
  • Flowrates: 1µl/min to 6 mL/min
Integrated Degasser

  • 2-channel degasser
  • Internal volume only 285 µl
  • Eliminates baseline fluctuations, advanced error and leak checking functions

Wetted parts:

  • Instrument: FEP, PEEK, Systec AF, FFKM
  • Flow-cell: PDMS/PEEK/titanium
Dimensions  62 x 41 x 47 cm (24.5" x 16" x 18.5")
Weight  30 kg (66 lbs)
Shipping volumetric weight 50 kg (110 lbs)

LIQUID HANDLING SPR Navi™ 210A

Liquid handling Integrated Autosampler The instrument is equipped with one autosampling areas:

  • Autosampling area:
    •  Fully configurable program
    • Sample capacity: 6 samples in vials or bottles
    • Injection modes: Full loop, partial loop
    • Wash: Programmable, wash between injections and wash between vials
    • Loop volume: 12 - 2000 μL programmable, exchangable, others by request. Loops are standard liquid chromatography loops and can be exchanged easily.
Integrated Syringe pump

  • Sample syringe: 2 500 µL, 1-channel
  • Measurement flow (running buffer)
  • 2-channel
  • 2500 μL standard
  • Flowrates: 1µl/min to 6 mL/min

Integrated Degasser

  • 2-channel degasser
  • Internal volume only 285 µl
  • Eliminates baseline fluctuations, advanced error and leak checking functions

Wetted Parts

  • Instrument: FEP (teflon-like), PEEK, Systec AF (teflon-like), FFKM
  • Flow-cell: PDMS/PEEK/titanium
DimensionsWeight W 45 x H 41 x D 51 cm (18" x 16" x 20") 22 kg (49 lbs)
Shipping volumetric weight 50 kg (110 lbs)

LIQUID HANDLING SPR NAVI™ 200

Liquid handling Pump: Integrated 2-channel peristaltic pump, software controlled, 10-400 µL/min
Sample loading: Manual
Loop volume: 100 µL (50 µL, 250 µL and others available)
Injection: 12-port injector (manual switch)
For experiments with liquids, we strongly recommend a degasser.The instrument can be equipped with a syringe pump or upgraded into SPR Navi 210A or 220A.Wetted parts:

  • Instrument: PEEK
  • Flow-cell: PDMS/PEEK/titanium
Dimensions W 32 x H 40 x D34 cm  (15" x 12.5" x 12.4")
Weight 12 kg (22 lbs)
Shipping volumetric weight 50 kg (110 lbs)

 

Brochures

MP-SPR: A unique technique to characterize nanoscale surfaces and interfaces
6-page Brochure for Material Sciences, 2013
pdf 1.4 MB Download
MP-SPR: From biomolecular interactions to biophysical and biomaterial studies!
6-page Brochure for Life Sciences, 2013
pdf 1.7 MB Download

Scientific Solutions

Scientific Solutions have been providing carefully selected laboratory and analytical equipment to Australian Industries such as Environmental, Pharmaceutical, Food and Beverage and Research markets of the Science Industry for over a decade.

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