SPR NAVI™ 220A - THE AUTOMATED MP-SPR

SPR NAVI™ 220A – THE AUTOMATED MP-SPR

SPR Navi™ 220A is the top of our line. Choose SPR Navi™ 220A, if you want to perform high-throughput MP-SPR with minimum dead-volumes and increased measurement confidence. As shown in the comparison, it is fully automated. Also here, to fully enjoy the MP-SPR, we recommend the additional set of lasers.

Product Category: SPR

Industry: Life Sciences, Particle and surface science

Supplier: Bionavis

Description
Specifications

Description

SPR NAVI™ 220A - THE AUTOMATED MP-SPR

Key Features of SPR Navi™ 220A:

True Goniometric SPR

Unlike most SPR systems, SPR Navi™ 220A is a true goniometric SPR. This eanbles a wide angular scan range (= 40–78°) that produces a complete SPR curve with absolute angle information. The true goniometric SPR configuration confers great advantages as it widens the range of refractive indexes at which you can measure, allowing you to characterise surfaces in gas and/or liquid. It also allows you to check the quality of user-defined surfaces before sample injection, providing you with additional evidences. In other words, the wide angular scan range makes SPR Navi 200 an extremely sensitive and versatile characterization instrument.

Customizable flow cell

SPR Navi™ 220A comes with dual measurement channels for in-line referencing or duplicate measurements. In addition, the easily accessible flow-cell can be customized for user-specific experiments, thus broadening your experimental capabilities. For instance, BioNavis can provide electrochemical flow cells or flow cells equipped with optical fibers for surface illumination. The flow cell shape can also be customized to tailor the flow dynamics to your needs.

Flexible sensor-slide system

With the SPR Navi™ 220A instrument, you can use the innovative sensor-slide holder for easy “drop-in” placement of your sensor-slide into the instrument. It allows users to change surfaces within seconds, making SPR Navi™ 220A an extremely convenient instrument to work with. The sensor-slide holders can be labeled and used for long-term storage of your custom-modified surfaces. Sensor-slides can be modified outside the instrument, with a great number and combination of layers, prior to the actual analysis. This unique removable sensor system provides additional flexibility. It allows you to combine SPR data with other widely used techniques to provide supporting information, including surface topography, surface wettability, chemical composition, photochemical processes, swelling, adsorption...

Contamination free MP-SPR

The proprietary optical elastomer in the SPR Navi™ 220A finally does away with the need for messy and potentially contaminating index-matching oil. The result is a contamination-free environment for your measurements. The optional immobilizer, a horizontally oriented stand-alone flow cell, can be used for in-situ functionalization of SPR slides. This further minimizes the risk of cross-contamination and enables minimal sample depositions.

Specifications

SPR NAVI™ SPECIFICATIONS

ANALYTICAL PERFORMANCE
Measured property	SPR Navi™ instruments measure light intensity reflected from the sensor surface (absolute measurement), while traditional SPR instruments measure light shift (only a relative measurement).
Refractive index (RI) range	1.00 - 1.40 (Can be extended using additional wavelength option) Using the same optics (prism and sensor slides) and in the same automated scan.
Dynamic range	38 000 mdeg
Noise level	0.3 µRIU (short term) 1 µRIU corresponds to 1 pg/mm² surface coverage of a typical protein. 100-fold improvement using Selectively Amplified Surface Plasmon Resonance.
Working range	kon = 10E3 < ka < 10E8 M-1 s-1 koff = 0.1 < kd < 10E-7 s-1 KD = 10E-3 - 10E-12 M-1
Fastest sampling rate	from 4 ms in Fixed Angle advanced timing mode
Temperature control	15-45oC (ambient +20/-7oC)

Note on working range: Values ka and kd are also dependent of each other, as well as the Bmax (binding maximum) values of the assay at the time, so in principle it is completely sample dependent range and the given ranges are just a guideline.

Extending working range: The range can be extended with careful experimental design. Please refer to J. Mol. Recognit. 2005; 18: 307–317 for more information.
Another option to extend the working range is Selectively Amplified Surface Plasmon Resonance (SAMP-SPR) technology that can be applied with SPR Navi™ instruments having additional wavelength (785 nm). SAMP-SPR is ideally suited to measure low concentrations of analyte in complex biological mixtures, with 100 times increased sensitivity and significantly reduced interference from background non-specific binding. This technology can be used to extend the range of affinity.

INSTRUMENT
Technique	Multi-Parametric Surface Plasmon Resonance (MP-SPR)
Measuring principle	True goniometric SPR with rotating laser (true angluar resolution 0.001o)
Wavelength of light	670 nm (and 785 nm with additional wavelength option) (other wavelengths available by request)
Optical channels	2
SPR angle scaning range	40o - 78o (with the same optics and same sensor slides and in the same scan, no need for prism exchange!, no manual manipulation needed)
Prism coating	Optical gel index matching (no need for messy oil operation between measurements)
Sensor slides	Au, Pt, Al, TiO2, SiO2, CMD, ... 12 x 20 mm
Refractive index (RI) range for the running buffer/bulk	1.00 - 1.45
Dynamic range	38 000 mdeg
Temperature of measurement chamber	15-45oC (ambient +20/-7oC) (in all models)
Temperature of sample storage	4-40°C at 25°C ambient temperature (only in 220A model!)
Computer requirements	The minimum PC requirements are: MS Windows® XP / VISTA / 7 (not Starter editions used in netbooks) both 32- and 64-bit versions are supported 1xUSB 2.0 2 GB RAM 2 Gb HD
Power requirements	100W, 100-240V, 50/60Hz

LIQUID HANDLING SPR NAVI™ 220A

	Liquid handling	Integrated Autosampler The instrument is equipped with two autosampling areas, one for small volumes and another for large volumes: Autosampling area 1: Fully configurable program Sample capacity: 1 microtiter plate (96 wells, both high and low format), 48 vials (1.5 mL, optional), or 12 vials (10 mL, optional) Injection modes: Full loop, partial loopfill Loop volume: 12 - 2000 μL programmable, exchangable, others by request Temperature controlled sample storage: 4-40°C Autosampling area 2: Fully configurable program Sample capacity: 6 samples in vials or bottles Injection modes: Full loop Wash: Programmable, wash between injections and wash between vials Loop volume: 12 - 2000 μL programmable, exchangable, others by request. Loops are standard liquid chromatography loops and can be exchanged easily.
		Integrated Syringe pump Sample syringe: 2 500 µL, 1-channel Measurement flow (running buffer) 2-channel 2500 μL standard Flowrates: 1µl/min to 6 mL/min
		Integrated Degasser 2-channel degasser Internal volume only 285 µl Eliminates baseline fluctuations, advanced error and leak checking functions Wetted parts: Instrument: FEP, PEEK, Systec AF, FFKM Flow-cell: PDMS/PEEK/titanium
	Dimensions	62 x 41 x 47 cm (24.5" x 16" x 18.5")
	Weight	30 kg (66 lbs)
	Shipping volumetric weight	50 kg (110 lbs)

LIQUID HANDLING SPR Navi™ 210A

	Liquid handling	Integrated Autosampler The instrument is equipped with one autosampling areas: Autosampling area: Fully configurable program Sample capacity: 6 samples in vials or bottles Injection modes: Full loop, partial loop Wash: Programmable, wash between injections and wash between vials Loop volume: 12 - 2000 μL programmable, exchangable, others by request. Loops are standard liquid chromatography loops and can be exchanged easily.
		Integrated Syringe pump Sample syringe: 2 500 µL, 1-channel Measurement flow (running buffer) 2-channel 2500 μL standard Flowrates: 1µl/min to 6 mL/min Integrated Degasser 2-channel degasser Internal volume only 285 µl Eliminates baseline fluctuations, advanced error and leak checking functions Wetted Parts Instrument: FEP (teflon-like), PEEK, Systec AF (teflon-like), FFKM Flow-cell: PDMS/PEEK/titanium

	DimensionsWeight	W 45 x H 41 x D 51 cm (18" x 16" x 20") 22 kg (49 lbs)
	Shipping volumetric weight	50 kg (110 lbs)

LIQUID HANDLING SPR NAVI™ 200

	Liquid handling	Pump: Integrated 2-channel peristaltic pump, software controlled, 10-400 µL/min
		Sample loading: Manual Loop volume: 100 µL (50 µL, 250 µL and others available) Injection: 12-port injector (manual switch) For experiments with liquids, we strongly recommend a degasser.The instrument can be equipped with a syringe pump or upgraded into SPR Navi 210A or 220A.Wetted parts: Instrument: PEEK Flow-cell: PDMS/PEEK/titanium
	Dimensions	W 32 x H 40 x D34 cm (15" x 12.5" x 12.4")
	Weight	12 kg (22 lbs)
	Shipping volumetric weight	50 kg (110 lbs)

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