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MP-SPR Navi™ 200 OTSO

Semi-automated and modular 

MP-SPR Navi™ 200 OTSO is our entry level MP-SPR system making it ideally suited for teaching and educational purposes. Open design is optimal when auxiliary instruments are to be used.

Product Category: SPR

Industry: Life Sciences, Particle and surface science

Supplier: Bionavis

Description

Features and benefits

  • Exceptionally  wide  angular  range 40˚ – 78˚ enables  measurements  of small  organic  molecules  and  large  proteins,  but  also  metallic nanoparticles and cells. One scan encompasses both environments: gas and liquid and enables to separate bulk effect in line using PureKinetics™
  • Parallel injections to 2 fluidic channels
  • Controlled liquid flow conditions with peristaltic pump
  • Manual filling of injection loops required
  • Optionally instrument can be equipped with up to 4 different wavelengths measured from each liquid channel enabling detection of thickness and refractive index or conformation changes
  • 200 OTSO is sold with a basic software package of MP-SPR  Navi™ Control and DataViewer for convenient control of the instrument and basic analysis of the results. Advanced analysis modules are optional: MP-SPR Navi™ LayerSolver to determine thickness and RI from multiple wavelength measurements and TraceDrawer for  MP-SPR  Navi™ – a powerful  software  for  the determination  of affinity, kinetic constants and more
  • MP-SPR Navi™ instruments utilize optical RI matching elastomer and therefore, sensors are very easy to exchange and remove for  further analysis with other methods such as AFM or microscopy
  • Open design enables convenient access to the easily removable flow cell, as well as to tubing, pump and injection valves
  • Complement  with electrochemistry, high chemical resistant and gas flow cells as well as fluorescence setup provides combined or validation measurements
  • The instrument enables attachment of a separate autosampler, pump, degasser or even own microfluidic sample preprocessing cartridge

Specifications

SPR Navi™ FLOW-CELLS

Flow-cell type Inlet diameter Number of fluidic channels Number of optical channels Material Volume of one channel
SPR301 
Standard flow-cell for SPR Navi 220A
medium 2 2 polydimethylsiloxane (PDMS) 1 µL
SPR302-LS
Flow-cell for fast kinetic studies
small 2 2  polyetheretherketone
(PEEK)
1 µL
SPR305-MS
Standard flow-cell for SPR Navi 210A,
optimal flow-cell for material science measurements
large 2 2 PEEK 1 µL
SPR306-TI
High chemical resistance
large 2 2 Titanium and Kalrez material 1 µL
SPR310-GS
Gas flow-cell
2 2 PDMS
(Titanium optional)
1 µL or 5 µL (depending on application)
SPR321-EC
Electrochemical flow-cell
1 2 PEEK, NBR 70/ Kalrez 100 µL
SPR330-HR
High Chemical Resistance flow-through
cuvette
1 2 PEEK, Kalrez 100 µL
SPR305-Cust
Custom made flow-cell
custom diameter 1 / 2 2 PEEK, Kalrez 1 µL / 100 µL
SPR323-Cust
Custom made flow-cell with additional
optical access e.g. fluorescence

Specifications are subject to change without prior notice.

All flow-cells can be easily exchanged by the user. All flow-cells enable measurements in air and in liquid, but only gas flow-cell enables measurements with pressurized gas.

 


SPR NAVI™ SPECIFICATIONS

ANALYTICAL
PERFORMANCE
Measured property SPR Navi™ instruments measure light intensity reflected from the sensor surface (absolute measurement), while traditional SPR instruments measure light shift (only a relative measurement).
Refractive index (RI) range 1.00 - 1.40 (Can be extended using additional wavelength option)
Using the same optics (prism and sensor slides) and in the same automated scan.
Dynamic range 38 000 mdeg
Noise level 0.3 µRIU (short term)
1 µRIU corresponds to 1 pg/mm² surface coverage of a typical protein.
100-fold improvement using Selectively Amplified Surface Plasmon Resonance.
Working range kon = 10E3 < ka < 10E8 M-1 s-1
koff = 0.1 < kd < 10E-7 s-1
KD = 10E-3 - 10E-12 M-1
Fastest sampling rate from 4 ms
in Fixed Angle advanced timing mode
Temperature control 15-45oC  (ambient +20/-7oC)

Note on working range: Values ka and kd are also dependent of each other, as well as the Bmax (binding maximum) values of the assay at the time, so in principle it is completely sample dependent range and the given ranges are just a guideline.

Extending working range: The range can be extended with careful experimental design. Please refer to J. Mol. Recognit. 2005; 18: 307–317 for more information.
Another option to extend the working range is Selectively Amplified Surface Plasmon Resonance (SAMP-SPR) technology that can be applied with SPR Navi™ instruments having additional wavelength (785 nm). SAMP-SPR is ideally suited to measure low concentrations of analyte in complex biological mixtures, with 100 times increased sensitivity and significantly reduced interference from background non-specific binding. This technology can be used to extend the range of affinity.

INSTRUMENT
Technique Multi-Parametric Surface Plasmon Resonance (MP-SPR)
Measuring principle True goniometric SPR with rotating laser (true angluar resolution 0.001o)
Wavelength of light 670 nm (and 785 nm with additional wavelength option)
(other wavelengths available by request)
Optical channels 2
SPR angle scaning range 40o - 78o
(with the same optics and same sensor slides and in the same scan, no need for prism exchange!, no manual manipulation needed)
Prism coating Optical gel index matching
(no need for messy oil operation between measurements)
Sensor slides  Au, Pt, Al, TiO2, SiO2, CMD, ...
12 x 20 mm
Refractive index (RI) range for the running buffer/bulk 1.00 - 1.45
Dynamic range 38 000 mdeg
Temperature of measurement chamber 15-45oC  (ambient +20/-7oC) (in all models)
Temperature of sample storage 4-40°C at 25°C ambient temperature (only in 220A model!)
Computer requirements The minimum PC requirements are:
MS Windows® XP / VISTA / 7 (not Starter editions used in netbooks)
both 32- and 64-bit versions are supported
1xUSB 2.0
2 GB RAM
2 Gb HD
Power requirements 100W, 100-240V, 50/60Hz

LIQUID HANDLING SPR NAVI™ 220A

Liquid handling Integrated Autosampler The instrument is equipped with two autosampling areas, one for small volumes and another for large volumes:

  • Autosampling area 1:
    • Fully configurable program
    • Sample capacity: 1 microtiter plate (96 wells, both high and low format), 48 vials (1.5 mL, optional), or 12 vials (10 mL, optional)
    • Injection modes: Full loop, partial loopfill
    • Loop volume: 12 - 2000 μL programmable, exchangable, others by request
    • Temperature controlled sample storage: 4-40°C
  • Autosampling area 2:
    • Fully configurable program
    • Sample capacity: 6 samples in vials or bottles
    • Injection modes: Full loop
    • Wash: Programmable, wash between injections and wash between vials
    • Loop volume: 12 - 2000 μL programmable, exchangable, others by request. Loops are standard liquid chromatography loops and can be exchanged easily.
Integrated Syringe pump

  • Sample syringe: 2 500 µL, 1-channel
  • Measurement flow (running buffer)
  • 2-channel
  • 2500 μL standard
  • Flowrates: 1µl/min to 6 mL/min
Integrated Degasser

  • 2-channel degasser
  • Internal volume only 285 µl
  • Eliminates baseline fluctuations, advanced error and leak checking functions

Wetted parts:

  • Instrument: FEP, PEEK, Systec AF, FFKM
  • Flow-cell: PDMS/PEEK/titanium
Dimensions  62 x 41 x 47 cm (24.5" x 16" x 18.5")
Weight  30 kg (66 lbs)
Shipping volumetric weight 50 kg (110 lbs)

LIQUID HANDLING SPR Navi™ 210A

Liquid handling Integrated Autosampler The instrument is equipped with one autosampling areas:

  • Autosampling area:
    •  Fully configurable program
    • Sample capacity: 6 samples in vials or bottles
    • Injection modes: Full loop, partial loop
    • Wash: Programmable, wash between injections and wash between vials
    • Loop volume: 12 - 2000 μL programmable, exchangable, others by request. Loops are standard liquid chromatography loops and can be exchanged easily.
Integrated Syringe pump

  • Sample syringe: 2 500 µL, 1-channel
  • Measurement flow (running buffer)
  • 2-channel
  • 2500 μL standard
  • Flowrates: 1µl/min to 6 mL/min

Integrated Degasser

  • 2-channel degasser
  • Internal volume only 285 µl
  • Eliminates baseline fluctuations, advanced error and leak checking functions

Wetted Parts

  • Instrument: FEP (teflon-like), PEEK, Systec AF (teflon-like), FFKM
  • Flow-cell: PDMS/PEEK/titanium
DimensionsWeight W 45 x H 41 x D 51 cm (18" x 16" x 20") 22 kg (49 lbs)
Shipping volumetric weight 50 kg (110 lbs)

LIQUID HANDLING SPR NAVI™ 200

Liquid handling Pump: Integrated 2-channel peristaltic pump, software controlled, 10-400 µL/min
Sample loading: Manual
Loop volume: 100 µL (50 µL, 250 µL and others available)
Injection: 12-port injector (manual switch)
For experiments with liquids, we strongly recommend a degasser.The instrument can be equipped with a syringe pump or upgraded into SPR Navi 210A or 220A.Wetted parts:

  • Instrument: PEEK
  • Flow-cell: PDMS/PEEK/titanium
Dimensions W 32 x H 40 x D34 cm  (15" x 12.5" x 12.4")
Weight 12 kg (22 lbs)
Shipping volumetric weight 50 kg (110 lbs)

 

Videos

See how to measure thickness and refractive index of thin inorganic layers using MP-SPR!

Thin metal films from 3.7 Ångströms up to 100 nanometers thick can be measured. Transparent films can be measured up tens of micrometers in thickness. Besides thickness and refractive index, MP-SPR measures also interactions with different gas or liquid samples, including ethanol or toluene vapors, solvents, but also plasma and serum. This allows real-time measurements of antifouling capabilities of thin film coatings as well as measurements of biocompatibility.

Contact your local distribution partner or BioNavis for further information.

Real-time conformation changes can be measured for instance upon change of temperature, pH, electric potential or light trigger. MP-SPR Navi™ 200 OTSO is a semi-automated MP-SPR instrument with 2-channel fluidics. It can be combined with different techniques including electrochemistry and fluorescence. 200 OTSO can be equipped with up to 4 wavelengths in the same channel, allowing reliable measurements of thickness, refractive index and dispersion coefficient.

Put yourself in the shoes of a scientist with our GoPro movie.

https://bionavis.com/wp-content/uploads/2021/09/200-otso-thin-films-short-27042018.mp4?_=1

Whitepapers

Please see the Bionavis website for a series of application notes

 

https://www.bionavis.com/en/publication_type/application-notes/

Accessories

Flow-cell2

Flow-cells

We offer a wide variety of flow-cells suitable for specific applications. All flow-cells can be easily exchanged by the user.

Our standard flow-cells are available in a variety of materials (PDMS, PEEK, Kalrez) depending on application such as kinetic studies or material characterization. Inlet diameter of the tubings and fluidic channel volume is optimized for each purpose.

Our special flow-cells enable the combination of MP-SPR measurements with electrochemistry or fluorescence and the measurement of live cells. All our flow-cells enable measurements in air and liquid and our special gas flow-cell enables even measurements with pressurized gas.

Contact us for more information and to find the most suitable flow-cell for your measurements!

Immobilizer

MP-SPR Navi™ Immobilizer

Our MP-SPR Navi™ Immobilizer is an especially designed stand-alone holder for chemical modifications and functionalization of MP-SPR Navi™ sensor surfaces. MP-SPR Navi™ Immobilizer is compatible with the MP-SPR Navi™ sensor slides and MP-SPR Navi™ sensor slide holder.

Read more about immobilizer in AN#130.

Contact us for more information on our MP-SPR Navi™ immobilizer.

We provide the only MP-SPR instruments on the market that can be equipped with multiple wavelengths!

MP-SPR measures uniquely same surface spot at two different wavelengths which enables thickness and refractive index measurements. This feature is useful in all MP-SPR applications but especially with material characterization, biophysics, and live cell studies.

All MP-SPR Navi™ instruments can be equipped with up to 2 different wavelengths in each channel. MP-SPR Navi™ 200 OTSO, 210A VASA and 220A NAALI  instruments can be equipped with up to 4 different wavelengths in each channel.

Additional wavelength option should be selected together with the instrument.

Get most out of the feature and combine it with LayerSolver software.

If you already have MP-SPR Navi™ system, contact us to learn how to upgrade it with additional lasers!

We offer a wide range of sensor slides manufactured according to high quality standards

All BioNavis sensors are cut to 12х20 mm size to fit into the BioNavis sensor slide holder and thus sensors are compatible with all the SPR Navi™ and MP-SPR Navi™ instruments.

Please see the Bionavis website for the range and choose the best sensor surface for your measurements. https://www.bionavis.com/en/products/additional-options-and-consumables/sensor-slides/

BioNavis offers a variety of software for  data collection with our MP-SPR Navi™ instruments and analysis of MP-SPR data

https://www.bionavis.com/en/products/additional-options-and-consumables/software/

Scientific Solutions

Scientific Solutions have been providing carefully selected laboratory and analytical equipment to Australian Industries such as Environmental, Pharmaceutical, Food and Beverage and Research markets of the Science Industry for over a decade.

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