
SPR Navi200
Product Category: SPR
Industry: Life Sciences, Particle and surface science
Supplier: Bionavis
Description
SPR NAVI™ 200 - THE MODULAR MP-SPR
SPR Navi™ 200 is the core measurement unit in our product range. Choose SPR Navi™ 200, if you want to connect MP-SPR to other measurement instruments or if you want to start with a lite version of MP-SPR and upgrade it later on with our range of stand-alone accessories.
Key Features of SPR Navi™ 200:
Modularity
SPR Navi™ 200 allows you to connect other stand-alone instruments or tune it to your specific application needs. As shown in the comparison, it is semi-automatic by default, but can be automated using our stand-alone autosampler. Also here, to fully enjoy the MP-SPR, we recommend the additional set of lasers.
True Goniometric SPR
Unlike most SPR systems, SPR Navi™ 200 is a true goniometric SPR. This eanbles a wide angular scan range (= 40–78°) that produces a complete SPR curve with absolute angle information. The true goniometric SPR configuration confers great advantages as it widens the range of refractive indexes at which you can measure, allowing you to characterize surfaces in gas and/or liquid. It also allows you to check the quality of user-defined surfaces before sample injection, providing you with additional evidences. In other words, the wide angular scan range makes SPR Navi 200 an extremely sensitive and versatile characterization instrument.
Customizable flow cell
SPR Navi™ 200 comes with dual measurement channels for in-line referencing or duplicate measurements. In addition, the easily accessible flow-cell can be customized for user-specific experiments, thus broadening your experimental capabilities. For instance, BioNavis can provide electrochemical flow cells or flow cells equipped with optical fibers for surface illumination. The flow cell shape can also be customized to tailor the flow dynamics to your needs.
Flexible sensor-slide system
With the SPR Navi™ 200 System, you can use the innovative sensor-slide holder for easy “drop-in” placement of your sensor-slide into the instrument. It allows users to change surfaces within seconds, making SPR Navi™ 200 an extremely convenient instrument to work with. The sensor-slide holders can be labeled and used for long-term storage of your custom-modified surfaces. Sensor-slides can be modified outside the instrument, with a great number and combination of layers, prior to the actual analysis. This unique removable sensor system provides additional flexibility. It allows you to combine SPR data with other widely used techniques to provide supporting information, including surface topography, surface wettability, chemical composition, photochemical processes, swelling, adsorption...
Contamination free MP-SPR & open design
The proprietary optical elastomer in the SPR Navi™ 200 finally does away with the need for messy and potentially contaminating index-matching oil. In addition, SPR Navi™ 200 open design enables convenient access to the easily removable flow cell, as well as to tubing, pump and injection valve. The result is a contamination-free environment for your measurements as well as an open platform for easy cleaning and customizations. The optional immobilizer, a horizontally oriented stand-alone flow cell, can be used for in-situ functionalization of SPR slides. This further minimizes the risk of cross-contamination and enables minimal sample depositions.
Specifications
SPR Navi™ FLOW-CELLS
Flow-cell type | Inlet diameter | Number of fluidic channels | Number of optical channels | Material | Volume of one channel |
SPR301 Standard flow-cell for SPR Navi 220A |
medium | 2 | 2 | polydimethylsiloxane (PDMS) | 1 µL |
SPR302-LS Flow-cell for fast kinetic studies |
small | 2 | 2 | polyetheretherketone (PEEK) |
1 µL |
SPR305-MS Standard flow-cell for SPR Navi 210A, optimal flow-cell for material science measurements |
large | 2 | 2 | PEEK | 1 µL |
SPR306-TI High chemical resistance |
large | 2 | 2 | Titanium and Kalrez material | 1 µL |
SPR310-GS Gas flow-cell |
2 | 2 | PDMS (Titanium optional) |
1 µL or 5 µL (depending on application) | |
SPR321-EC Electrochemical flow-cell |
1 | 2 | PEEK, NBR 70/ Kalrez | 100 µL | |
SPR330-HR High Chemical Resistance flow-through cuvette |
1 | 2 | PEEK, Kalrez | 100 µL | |
SPR305-Cust Custom made flow-cell |
custom diameter | 1 / 2 | 2 | PEEK, Kalrez | 1 µL / 100 µL |
SPR323-Cust Custom made flow-cell with additional optical access e.g. fluorescence |
Specifications are subject to change without prior notice.
All flow-cells can be easily exchanged by the user. All flow-cells enable measurements in air and in liquid, but only gas flow-cell enables measurements with pressurized gas.
SPR NAVI™ SPECIFICATIONS
ANALYTICAL PERFORMANCE |
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Measured property | SPR Navi™ instruments measure light intensity reflected from the sensor surface (absolute measurement), while traditional SPR instruments measure light shift (only a relative measurement). |
Refractive index (RI) range | 1.00 - 1.40 (Can be extended using additional wavelength option) Using the same optics (prism and sensor slides) and in the same automated scan. |
Dynamic range | 38 000 mdeg |
Noise level | 0.3 µRIU (short term) 1 µRIU corresponds to 1 pg/mm² surface coverage of a typical protein. 100-fold improvement using Selectively Amplified Surface Plasmon Resonance. |
Working range | kon = 10E3 < ka < 10E8 M-1 s-1 koff = 0.1 < kd < 10E-7 s-1 KD = 10E-3 - 10E-12 M-1 |
Fastest sampling rate | from 4 ms in Fixed Angle advanced timing mode |
Temperature control | 15-45oC (ambient +20/-7oC) |
Note on working range: Values ka and kd are also dependent of each other, as well as the Bmax (binding maximum) values of the assay at the time, so in principle it is completely sample dependent range and the given ranges are just a guideline.
Extending working range: The range can be extended with careful experimental design. Please refer to J. Mol. Recognit. 2005; 18: 307–317 for more information.
Another option to extend the working range is Selectively Amplified Surface Plasmon Resonance (SAMP-SPR) technology that can be applied with SPR Navi™ instruments having additional wavelength (785 nm). SAMP-SPR is ideally suited to measure low concentrations of analyte in complex biological mixtures, with 100 times increased sensitivity and significantly reduced interference from background non-specific binding. This technology can be used to extend the range of affinity.
INSTRUMENT | |
Technique | Multi-Parametric Surface Plasmon Resonance (MP-SPR) |
Measuring principle | True goniometric SPR with rotating laser (true angluar resolution 0.001o) |
Wavelength of light | 670 nm (and 785 nm with additional wavelength option) (other wavelengths available by request) |
Optical channels | 2 |
SPR angle scaning range | 40o - 78o (with the same optics and same sensor slides and in the same scan, no need for prism exchange!, no manual manipulation needed) |
Prism coating | Optical gel index matching (no need for messy oil operation between measurements) |
Sensor slides | Au, Pt, Al, TiO2, SiO2, CMD, ... 12 x 20 mm |
Refractive index (RI) range for the running buffer/bulk | 1.00 - 1.45 |
Dynamic range | 38 000 mdeg |
Temperature of measurement chamber | 15-45oC (ambient +20/-7oC) (in all models) |
Temperature of sample storage | 4-40°C at 25°C ambient temperature (only in 220A model!) |
Computer requirements | The minimum PC requirements are: MS Windows® XP / VISTA / 7 (not Starter editions used in netbooks) both 32- and 64-bit versions are supported 1xUSB 2.0 2 GB RAM 2 Gb HD |
Power requirements | 100W, 100-240V, 50/60Hz |
LIQUID HANDLING SPR NAVI™ 220A
Liquid handling | Integrated Autosampler The instrument is equipped with two autosampling areas, one for small volumes and another for large volumes:
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Integrated Syringe pump
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Integrated Degasser
Wetted parts:
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Dimensions | 62 x 41 x 47 cm (24.5" x 16" x 18.5") | |
Weight | 30 kg (66 lbs) | |
Shipping volumetric weight | 50 kg (110 lbs) |
LIQUID HANDLING SPR Navi™ 210A
Liquid handling | Integrated Autosampler The instrument is equipped with one autosampling areas:
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Integrated Syringe pump
Integrated Degasser
Wetted Parts
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DimensionsWeight | W 45 x H 41 x D 51 cm (18" x 16" x 20") 22 kg (49 lbs) | |
Shipping volumetric weight | 50 kg (110 lbs) |
Liquid handling | Pump: Integrated 2-channel peristaltic pump, software controlled, 10-400 µL/min | |
Sample loading: Manual Loop volume: 100 µL (50 µL, 250 µL and others available) Injection: 12-port injector (manual switch) For experiments with liquids, we strongly recommend a degasser.The instrument can be equipped with a syringe pump or upgraded into SPR Navi 210A or 220A. Wetted parts:
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Dimensions | W 32 x H 40 x D34 cm (15" x 12.5" x 12.4") | |
Weight | 12 kg (22 lbs) | |
Shipping volumetric weight | 50 kg (110 lbs) |
Videos
VIDEO: INTRODUCTION TO SPR NAVI™ 200
See, how easy it is to operate SPR Navi 200!
The whole procedure, how to use SPR Navi 200, is shown in a video. All steps are clearly explained. The main phases of operation include:
- Preparation of the Instrument
- Preparation of Sensor Slides
- Sample Preparation
- Measurement
- Clean-Up
The video presents the lite version of SPR Navi instrumentation. SPR Navi 200 can be automated with a set of stand-alone devices, while SPR Navi 220A is fully automated. See the performance measures of SPR Navi 200!
SPR Navi instruments are THE ONLY devices based on Multi-Parametric Surface Plasmon Resonance (MP-SPR)! The applicability ranges from Life Sciences throughMaterial Science to Sensor Development.
See also other versions of this movie!
- Go back to the VIDEOS page
- Introduction to PhD Life with SPR Navi™ 200 (7 min)
- Introduction to PhD Life 1.0 (4 min), Trailer (1 min)
- Introduction to SPR Navi™ 200 (4 min), Trailer (1 min)
Brochures
MP-SPR: A unique technique to characterize nanoscale surfaces and interfaces 6-page Brochure for Material Sciences, 2013 |
pdf 1.4 MB | Click To Download | |
MP-SPR: From biomolecular interactions to biophysical and biomaterial studies! 6-page Brochure for Life Sciences, 2013 |
pdf 1.7 MB | Click To Download | |