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Pressurized Fluid Extraction Food & Environmental

Pressurized Fluid Extraction (PFE) or Pressurized Liquid Extraction (PLE) is a new sample extraction method that employs liquid solvents at elevated temperatures and pressures to prepare samples for analysis by either gas chromatography or liquid chromatography. Pressurized liquid extraction is similar to Soxhlet extraction, except that during the extraction process the solvent condition inside the PLE cell approaches the supercritical region which results in more efficient extractions. The elevated temperature allows the sample to become more soluble and achieve a higher diffusion rate while the elevated pressure keeps the solvent below its boiling point. At elevated pressures and temperatures solvents can penetrate solid samples more efficiently which reduces solvent usage. A pressurized liquid extraction when compared to a traditional Soxhlet extraction shows a reduction in extraction time to 22 minutes from 18 hours and a decrease of total organic solvent consumption to 80 mL or less of organic solvent from 300 mL. To perform a pressurized liquid extraction, between 5 grams and 100 grams of sample is mixed with sodium sulfate, loaded in the extraction cell and capped with two filtration end fittings. The PLE™ system then automatically starts pressurizing and heating the samples. The pressure is maintained at 1500-3000 PSI, at a temperature of 70-200 ºC. The extracted solvent containing the target analytes is then automatically transferred to a concentration/evaporation vessel where it is brought to final volume directly in a gas chromatography (GC) or liquid chromatography (LC) vial. The vial can then be transferred to the analytical instrument for final analysis.

Product Category: Extraction/Concentration

Industry: Analytical and Environmental Labs, Food, Dairy and Wine

Supplier: FMS


Pressurized Liquid Extraction (PLE) System

The PLE® system is a high-speed pressurized liquid extraction system designed to perform sample extraction of multiple samples simultaneously. The PLE system delivers high recoveries and excellent precision for all analytes in minutes instead of hours. Inexpensive stainless steel extraction cells with end cap filtration keep operational costs at a minimum. Optional disposable end cap filtration increases productivity and saves valuable time.

Reduce Solvent Costs
Pressurized liquid extraction uses as little as 15 mL of solvent compared to more than 500 mL of solvent required to perform Soxhlet extractions.

Reduce Solvent Waste
The PLE system reduces solvent waste by using solvents more efficiently.

Increase Productivity
The entire extraction and cleanup may be performed in less than 30 minutes. Traditional methods can take 10 to 16 hours.

Reduce Operating Costs
Rapid extraction and clean-up, along with reduced solvent use and waste, reduces operating costs by as much as 70 percent.

Five to 250 mL Extraction Cell Sizes
The PLE™ system offers 5-250 mL low cost stainless steel extraction cells with Teflon endcap filtration. This wide range of extraction cell volumes allows the use of the same unit for all sample sizes, even in the same run.

Modular, Expandable and Affordable
The modular design of the PLE system allows you to purchase a one, two or three sample system to fit your budget. The system can be expanded to a five or six sample system as your application grows.

Eliminate cross contamination
Optional low-cost disposable extraction cells and Teflon filtration endcaps ensure trouble-free extraction and eliminate the risk of cross contamination.

One-Step Sample Extraction and Cleanup
The optional in-line cleanup module performs the entire sample extraction and cleanup in one step with increased speed and reduced cost.

Automatic Operation and Documentation
Real-time software allows six channels of pressure and six channels of temperature data to be plotted simultaneously. This powerful feature allows automatic documentation of all extraction data. Temperature and pressure data can be superimposed and printed in either graphic or tabular format and stored for future reference.

Modular Construction Provides for Easy Maintenance
The modular desgin of the PLE sytem combined with its exposed plumbing enables efficient system maintenance. The PLE system’s channels are designed to operate independently, so if one channel fails the others will continue to perform. This versatility ensures easy replacement with no down time.

Leak and Clog Free Operation
Simple design combined with large bore plumbing enables the PLE system to operate virtually leak and clog free.

Multiple Extractions
Programming of variable pressure and temperature allows extraction of a variety of compounds.


Technical Specification

Extraction cell size: 5 mL, 10 mL, 20 mL, 40 mL, 70 mL, 100 mL, 250 mL
Simultaneous Extractions 1 to 6 independent extraction modules for simultaneous extraction
Variable Cell Size per Simultaneous Extraction Run Run different cell sizes in the same simultaneous run 5 mL, 10 mL, 20 mL, 40 mL, 70 mL, 100 mL or 250 mL
Modular Design Individual Extraction Modules can be isolated from the system without interrupting the running system, no down time.
Automated Sample Cleanup Incell Sample Cleanup, Pre-packed Incell End Cap Sample Cleanup, Inline Column Sample Cleanup,
Interface to Automated Cleanup Systems Seamless programmable interface to PowerPrep™ Multi-Column Sample Cleanup System
Interface to Automated Concentration Systems Seamless Programmable interface to the SuperVap Concentrator to provide one step extraction and concentration or one step extraction, sample cleanup and concentration
Flow Rate 0 to 35 mL per minute
Pressure 0 to 2500 PSI
Temperature Ambient to 200 ºC
Solvents No limitation, mixing or selection of up to six different solvents
Collection Vessels Direct to a SuperVap Concentration Vessel or SuperVap Direct to GC Vial Concentration Vessel. All other standard collection vessels.
Automatic Documentation The DMS-6000 software allows multiple methods to be stored. Each parameter such as time, solvent volume, and final temperature can be set for each step. Pressure, temperature and dispensed volumes are displayed real time. All parameters for each run are documented and stored for future reference.
DMS 6000 Software  The PLE™ system uses a PC to control the entire extraction, sample cleanup and concentration process. The DMS-6000 real time software allows multiple samples to be processed simultaneously.
Recoveries and Reproducibility Method compliance or better
Real Time Monitoring, Plotting of Pressure and Temperature  


The DMS-6000 real time software allows six channels of pressure and six channels of temperature to be plotted in real time. This powerful feature allows automatic documentation of the entire extraction data. The temperature and pressure can be superimposed and printed in graphic or tabular format.
One Step Automated Extraction, Sample Clean up and Concentration Control Software Used to write, and edit extraction protocols, monitor, and store the extraction, ample cleanup and concentration data. It also controls the entire sample prep process.
Automatic Extraction Data Report  The DMS-6000 real time software allows the creation of an automatic data report based on the sample sequence set up. Printed data contains the graph and parameters of the sample extraction.


Weights:PLE Pump module = 10 lbs

PLE Control module = 15 lbs

PLE valve module = 15 lbs

PLE structure = 27 lbs

PLE Large cabinet = 18 lbs

PLE small cabinet = 24 lbs

PC = 21 lbs

Monitor = 10 lbs

Weights:Small cart = 35 lbs

Small tray = 20 lbs

Medium cart = 40 lbs

Medium tray = 25 lbs

Large cart = 45 lbs

Large tray = 35 lbs


Small cabinet + pump module + control module = 50 lbsLarge cabinet + valve module + structure = 60 lbs

PC + monitor = 30 lbs

PLE1 = 110 lbs + 30 lbs = 140 lbs

PLE2 = 170 lbs + 30 lbs = 200 lbs

PLE3 = 230 lbs + 30 lbs = 260 lbs

PLE4 = 290 lbs + 30 lbs = 320 lbs

PLE5 = 350 lbs + 30 lbs = 380 lbs

PLE6 = 410 lbs + 30 lbs = 440 lbs

Dimensions:PLE1 = 15” (38 cm) W X 35” (90 cm) H X 18” (45 cm) D

PLE2 = 23” (58 cm) W X 35” (90 cm) H X 18” (45 cm) D

PLE3 = 31” (78 cm) W X 35” (90 cm) H X 18” (45 cm) D

PLE4 = 39” (98 cm) W X 35” (90 cm) H X 18”(45 cm) D

PLE5 = 47” (118 cm) W X 35” (90 cm) H X 18” (45 cm) D

PLE6 = 55” (138 cm) W X 35” (90 cm) H X 18” (45 cm) D


Dimensions with cart and tray:PLE2 = 37” (94 cm) W X 64” (162 cm) H X 30” (75 cm) D

PLE3 = 45” (114 cm) W X 64” (162 cm) H X 30” (75 cm) D

PLE4 = 64” (162 cm) W X 64” (162 cm) H X 30” (75 cm) D

PLE5 = 64” (162 cm) W X 64” (162 cm) H X 30” (75 cm) D

PLE6 = 64” (162 cm) W X 64” (162 cm) H X 30” (75 cm) D




Pesticide Residue Analysis Using Pressurized Liquid Extraction for the Analysis of Pesticides in Food by GC/MS

A Quick Screen Method for the Extraction, Cleanup and Concentration of Toxaphene in Fish

Dioxins, PCBs and PBDEs in Human Serum Using Automated Pressurized Liquid Extraction, Multi-Column Cleanup, and Concentration

Extraction of Antioxidants from High Density Polyethylene (HDPE) By Pressurized Liquid Extraction (PLE)

One Step Extraction, Cleanup and Direct-to-Vial Concentration For PCBs in Soil and Sediment

One Step Extraction, Cleanup and Concentration of Pesticides from Soil

One Step Extraction, Cleanup and Concentration of Chlorinated Pesticides in Raw Coffee Beans

Pressurized Liquid Extraction of Soil Samples for the Analysis of Diesel Range Organics by GC/FID

Total Extractable Fat Using Pressurized Liquid Extraction (PLE)

The Analysis of Chlorinated Dioxins using Automated Pressurized Liquid Extraction, Multicolumn Cleanup and Concentration

Method for the Validation of PAHs in Soil and Sediment Samples Using Pressurized Liquid Extraction and Automated Cleanup

The Analysis of Polychlorinated Dibenzo-p-dioxins, Furans and Biphenyls in River Sediment with Automated Extraction and Clean Up

Use of Pressurized Liquid Extraction and Automated Column Chromatography in the Analysis of Polychlorinated Dibenzo-p-dioxins, Furans and Biphenyls in Human Serum

The Analysis of Polychlorinated Dibenzo-p-dioxins and Furans in Cranberry Pulp with Automated Extraction and Reduced Solvent Volume Clean Up

Polychlorinated Dibenzo-p-dioxins, Furans and Biphenyls in Fish Automated Sample Processing

Analysis of Soil for Polychlorinated Dibenzo-p-dioxins, Furans and Biphenyls using Pressurized Liquid Extraction and Automated Clean Up

The Analysis of Chlorinated Dioxins, Difurans in Pet Food

The Analysis of Polychlorinated Biphenyls in Coffee using Automated Extraction and Reduced Solvent Volume Clean Up

Automated Extraction and Cleanup of Egg Yolk Dioxins and Furans

Analyzing Infant Formula for Dioxins and Furans using Pressurized Liquid Extraction and Automated Cleanup

Automated Extraction and Clean Up of Crab Meat Dioxins and Furans

Dioxins, Furans, and PCBs in Peanut Butter Samples Processed with Automated Extraction and Clean Up

Analyzing Freeze-Dried and Regular Milk for Dioxins and Furans using Pressurized Liquid Extraction and Automated Clean Up

One Step Extraction, Cleanup and Concentration for PBDEs in Soil

Automated Extraction and Clean Up of Wood Boiler Ash Samples Dioxins, Furans, and PCBs

Scientific Solutions

Scientific Solutions have been providing carefully selected laboratory and analytical equipment to Australian Industries such as Environmental, Pharmaceutical, Food and Beverage and Research markets of the Science Industry for over a decade.

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