MP-SPR Navi™ 210A VASA

6-sample automation not only for material characterization!

Wider inner diameter of the flow-cell tubings and 6-sample automation makes MP-SPR Navi™ 210A VASA ideal for material characterization and nanoparticle research.

Product Category: SPR

Industry: Life Sciences, Particle and surface science

Supplier: Bionavis

Description

Features and benefits

Automated liquid handling of up to 6 different samples for unattended runs
Software switch for serial and parallel injections in 2 fluidic channels
Partial loop injections enabling minimized sample consumption
Exceptionally wide angular range 40˚ – 78˚ enables measurements of small organic molecules and large proteins, but also metallic nanoparticles and cells. One scan encompasses both environments: gas and liquid and enables in-line bulk effect separation providing PureKinetics™
Two independent wavelength measurements in each fluidic channel, enabling detection of thickness and refractive index or conformation changes
Optionally the instrument can be equipped with up to 4 different wavelengths measured from each liquid channel for optimal data for layer characterization
Controlled buffer flow conditions with precise syringe pumps and integrated degasser
Software package for convenient control of the instrument and easy analysis of the results. 210A VASA comes with MP-SPR Navi™ LayerSolver to determine thickness and RI from multiple wavelength measurements. TraceDrawer™ – a powerful software for determination of affinity and kinetic constants is optional
MP-SPR Navi™ instruments utilize optical RI matching elastomer and therefore, sensors are very easy to exchange and remove for further analysis with other methods such as AFM or microscopy
Open design enables convenient access to the easily removable flow cell, as well as to tubing, pump and injection valves
Complement with electrochemistry, high chemical resistant and gas flow cells as well as fluorescence setup provides combined or validation measurements

Specifications

SPR Navi™ FLOW-CELLS

Flow-cell type	Inlet diameter	Number of fluidic channels	Number of optical channels	Material	Volume of one channel
SPR301 Standard flow-cell for SPR Navi 220A	medium	2	2	polydimethylsiloxane (PDMS)	1 µL
SPR302-LS Flow-cell for fast kinetic studies	small	2	2	polyetheretherketone (PEEK)	1 µL
SPR305-MS Standard flow-cell for SPR Navi 210A, optimal flow-cell for material science measurements	large	2	2	PEEK	1 µL
SPR306-TI High chemical resistance	large	2	2	Titanium and Kalrez material	1 µL
SPR310-GS Gas flow-cell		2	2	PDMS (Titanium optional)	1 µL or 5 µL (depending on application)
SPR321-EC Electrochemical flow-cell		1	2	PEEK, NBR 70/ Kalrez	100 µL
SPR330-HR High Chemical Resistance flow-through cuvette		1	2	PEEK, Kalrez	100 µL
SPR305-Cust Custom made flow-cell	custom diameter	1 / 2	2	PEEK, Kalrez	1 µL / 100 µL
SPR323-Cust Custom made flow-cell with additional optical access e.g. fluorescence

Specifications are subject to change without prior notice.

All flow-cells can be easily exchanged by the user. All flow-cells enable measurements in air and in liquid, but only gas flow-cell enables measurements with pressurized gas.

SPR NAVI™ SPECIFICATIONS

ANALYTICAL PERFORMANCE
Measured property	SPR Navi™ instruments measure light intensity reflected from the sensor surface (absolute measurement), while traditional SPR instruments measure light shift (only a relative measurement).
Refractive index (RI) range	1.00 - 1.40 (Can be extended using additional wavelength option) Using the same optics (prism and sensor slides) and in the same automated scan.
Dynamic range	38 000 mdeg
Noise level	0.3 µRIU (short term) 1 µRIU corresponds to 1 pg/mm² surface coverage of a typical protein. 100-fold improvement using Selectively Amplified Surface Plasmon Resonance.
Working range	kon = 10E3 < ka < 10E8 M-1 s-1 koff = 0.1 < kd < 10E-7 s-1 KD = 10E-3 - 10E-12 M-1
Fastest sampling rate	from 4 ms in Fixed Angle advanced timing mode
Temperature control	15-45oC (ambient +20/-7oC)

Note on working range: Values ka and kd are also dependent of each other, as well as the Bmax (binding maximum) values of the assay at the time, so in principle it is completely sample dependent range and the given ranges are just a guideline.

Extending working range: The range can be extended with careful experimental design. Please refer to J. Mol. Recognit. 2005; 18: 307–317 for more information.
Another option to extend the working range is Selectively Amplified Surface Plasmon Resonance (SAMP-SPR) technology that can be applied with SPR Navi™ instruments having additional wavelength (785 nm). SAMP-SPR is ideally suited to measure low concentrations of analyte in complex biological mixtures, with 100 times increased sensitivity and significantly reduced interference from background non-specific binding. This technology can be used to extend the range of affinity.

INSTRUMENT
Technique	Multi-Parametric Surface Plasmon Resonance (MP-SPR)
Measuring principle	True goniometric SPR with rotating laser (true angluar resolution 0.001o)
Wavelength of light	670 nm (and 785 nm with additional wavelength option) (other wavelengths available by request)
Optical channels	2
SPR angle scaning range	40o - 78o (with the same optics and same sensor slides and in the same scan, no need for prism exchange!, no manual manipulation needed)
Prism coating	Optical gel index matching (no need for messy oil operation between measurements)
Sensor slides	Au, Pt, Al, TiO2, SiO2, CMD, ... 12 x 20 mm
Refractive index (RI) range for the running buffer/bulk	1.00 - 1.45
Dynamic range	38 000 mdeg
Temperature of measurement chamber	15-45oC (ambient +20/-7oC) (in all models)
Temperature of sample storage	4-40°C at 25°C ambient temperature (only in 220A model!)
Computer requirements	The minimum PC requirements are: MS Windows® XP / VISTA / 7 (not Starter editions used in netbooks) both 32- and 64-bit versions are supported 1xUSB 2.0 2 GB RAM 2 Gb HD
Power requirements	100W, 100-240V, 50/60Hz

LIQUID HANDLING SPR NAVI™ 220A

	Liquid handling	Integrated Autosampler The instrument is equipped with two autosampling areas, one for small volumes and another for large volumes: Autosampling area 1: Fully configurable program Sample capacity: 1 microtiter plate (96 wells, both high and low format), 48 vials (1.5 mL, optional), or 12 vials (10 mL, optional) Injection modes: Full loop, partial loopfill Loop volume: 12 - 2000 μL programmable, exchangable, others by request Temperature controlled sample storage: 4-40°C Autosampling area 2: Fully configurable program Sample capacity: 6 samples in vials or bottles Injection modes: Full loop Wash: Programmable, wash between injections and wash between vials Loop volume: 12 - 2000 μL programmable, exchangable, others by request. Loops are standard liquid chromatography loops and can be exchanged easily.
		Integrated Syringe pump Sample syringe: 2 500 µL, 1-channel Measurement flow (running buffer) 2-channel 2500 μL standard Flowrates: 1µl/min to 6 mL/min
		Integrated Degasser 2-channel degasser Internal volume only 285 µl Eliminates baseline fluctuations, advanced error and leak checking functions Wetted parts: Instrument: FEP, PEEK, Systec AF, FFKM Flow-cell: PDMS/PEEK/titanium
	Dimensions	62 x 41 x 47 cm (24.5" x 16" x 18.5")
	Weight	30 kg (66 lbs)
	Shipping volumetric weight	50 kg (110 lbs)

LIQUID HANDLING SPR Navi™ 210A

	Liquid handling	Integrated Autosampler The instrument is equipped with one autosampling areas: Autosampling area: Fully configurable program Sample capacity: 6 samples in vials or bottles Injection modes: Full loop, partial loop Wash: Programmable, wash between injections and wash between vials Loop volume: 12 - 2000 μL programmable, exchangable, others by request. Loops are standard liquid chromatography loops and can be exchanged easily.
		Integrated Syringe pump Sample syringe: 2 500 µL, 1-channel Measurement flow (running buffer) 2-channel 2500 μL standard Flowrates: 1µl/min to 6 mL/min Integrated Degasser 2-channel degasser Internal volume only 285 µl Eliminates baseline fluctuations, advanced error and leak checking functions Wetted Parts Instrument: FEP (teflon-like), PEEK, Systec AF (teflon-like), FFKM Flow-cell: PDMS/PEEK/titanium

	DimensionsWeight	W 45 x H 41 x D 51 cm (18" x 16" x 20") 22 kg (49 lbs)
	Shipping volumetric weight	50 kg (110 lbs)

LIQUID HANDLING SPR NAVI™ 200

	Liquid handling	Pump: Integrated 2-channel peristaltic pump, software controlled, 10-400 µL/min
		Sample loading: Manual Loop volume: 100 µL (50 µL, 250 µL and others available) Injection: 12-port injector (manual switch) For experiments with liquids, we strongly recommend a degasser.The instrument can be equipped with a syringe pump or upgraded into SPR Navi 210A or 220A.Wetted parts: Instrument: PEEK Flow-cell: PDMS/PEEK/titanium
	Dimensions	W 32 x H 40 x D34 cm (15" x 12.5" x 12.4")
	Weight	12 kg (22 lbs)
	Shipping volumetric weight	50 kg (110 lbs)

Videos

Put yourself in the shoes of a scientist with our GoPro movies!

Experiments are run on our MP-SPR Navi™ 210A VASA instrument. Samples are automatically handled from 6 positions and then run in series or in parallel over 2 fluidic channels. Thanks to multiple wavelengths and scanning angular scans, the instrument provides measurements of kinetic constants as well as provides layer properties.

The MP-SPR Navi instruments uniquely feature PureKinetics™, a function that allows in line measurements of bulk and thus resulting in uncompromised pure binding data.

Thickness and refractive index of polyelectrolyte multilayer

https://bionavis.com/wp-content/uploads/2021/09/210a-vasa-pem-short-ms-07122017.mp4?_=1

Binding affinity and kinetics of IgG – anti-IgG interaction

https://bionavis.com/wp-content/uploads/2021/09/210a-vasa-lgg-short-ls-31102017.mp4?_=2

Whitepapers

Please see the Bionavis website for a series of application notes

https://www.bionavis.com/en/publication_type/application-notes/

Accessories

Flow-cells

We offer a wide variety of flow-cells suitable for specific applications. All flow-cells can be easily exchanged by the user.

Our standard flow-cells are available in a variety of materials (PDMS, PEEK, Kalrez) depending on application such as kinetic studies or material characterization. Inlet diameter of the tubings and fluidic channel volume is optimized for each purpose.

Our special flow-cells enable the combination of MP-SPR measurements with electrochemistry or fluorescence and the measurement of live cells. All our flow-cells enable measurements in air and liquid and our special gas flow-cell enables even measurements with pressurized gas.

MP-SPR Navi™ Immobilizer

Our MP-SPR Navi™ Immobilizer is an especially designed stand-alone holder for chemical modifications and functionalization of MP-SPR Navi™ sensor surfaces. MP-SPR Navi™ Immobilizer is compatible with the MP-SPR Navi™ sensor slides and MP-SPR Navi™ sensor slide holder.

We provide the only MP-SPR instruments on the market that can be equipped with multiple wavelengths!

MP-SPR measures uniquely same surface spot at two different wavelengths which enables thickness and refractive index measurements. This feature is useful in all MP-SPR applications but especially with material characterization, biophysics, and live cell studies.

All MP-SPR Navi™ instruments can be equipped with up to 2 different wavelengths in each channel. MP-SPR Navi™ 200 OTSO, 210A VASA and 220A NAALI instruments can be equipped with up to 4 different wavelengths in each channel.

Additional wavelength option should be selected together with the instrument.

Get most out of the feature and combine it with LayerSolver software.

If you already have MP-SPR Navi™ system, contact us to learn how to upgrade it with additional lasers!

We offer a wide range of sensor slides manufactured according to high quality standards

All BioNavis sensors are cut to 12х20 mm size to fit into the BioNavis sensor slide holder and thus sensors are compatible with all the SPR Navi™ and MP-SPR Navi™ instruments.

Please see the Bionavis website for the range and choose the best sensor surface for your measurements. https://www.bionavis.com/en/products/additional-options-and-consumables/sensor-slides/

BioNavis offers a variety of software for data collection with our MP-SPR Navi™ instruments and analysis of MP-SPR data

https://www.bionavis.com/en/products/additional-options-and-consumables/software/

Scientific Solutions

Scientific Solutions have been providing carefully selected laboratory and analytical equipment to Australian Industries such as Environmental, Pharmaceutical, Food and Beverage and Research markets of the Science Industry for over a decade.

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