MP-SPR Navi™ 220A NAALI

Well plate automation for life sciences

MP-SPR Navi™ 220A NAALI measures not only interactions providing premium quality data with PureKinetics™, but also measures conformational changes and layer properties.

Product Category: SPR

Industry: Life Sciences, Particle and surface science

Supplier: Bionavis

Description

Features and benefits

Automation of samples from 96 and 384 well plates allows for unattended runs
Software switch for serial and parallel injections
Partial loop injections providing minimal sample consumption
Simultaneous measurements in 2 fluidic channels
Two independent wavelength measurements in each fluidic channel, enabling detection of thickness and refractive index or conformational changes
Instrument can be equipped optically with up to 4 different wavelenghts measured from each liquid channel for optimal data for layer characterization
Exceptionally wide angular range 40˚ – 78˚ enables measurements of small organic molecules and large proteins, but also metallic nanoparticles and cells. Most importantly, it enables in-line bulk effect separation with PureKinetics™
Measurements of kinetic constants, concentration and competition analysis, affinity and EC50
Controlled buffer flow conditions with precise syringe pumps and integrated degasser
Software package for convenient control of the instrument and easy analysis of the results. The instrument comes with TraceDrawer™ for MP-SPR Navi™ – a powerful software for the determination of affinity and kinetic constants and more. MP-SPR Navi™ LayerSolver for the determination of thickness and RI from multiple wavelength measurements is an optional module
MP-SPR Navi™ instruments utilize optical RI matching elastomer and therefore, sensors are very easy to exchange and remove for further analysis with other methods such as AFM or microscopy
Complement with electrochemistry, high chemical resistant and gas flow cells as well as fluorescence setup provides combined or validation measurements

Specifications

SPR NAVI™ SPECIFICATIONS

ANALYTICAL PERFORMANCE
Measured property	SPR Navi™ instruments measure light intensity reflected from the sensor surface (absolute measurement), while traditional SPR instruments measure light shift (only a relative measurement).
Refractive index (RI) range	1.00 - 1.40 (Can be extended using additional wavelength option) Using the same optics (prism and sensor slides) and in the same automated scan.
Dynamic range	38 000 mdeg
Noise level	0.3 µRIU (short term) 1 µRIU corresponds to 1 pg/mm² surface coverage of a typical protein. 100-fold improvement using Selectively Amplified Surface Plasmon Resonance.
Working range	kon = 10E3 < ka < 10E8 M-1 s-1 koff = 0.1 < kd < 10E-7 s-1 KD = 10E-3 - 10E-12 M-1
Fastest sampling rate	from 4 ms in Fixed Angle advanced timing mode
Temperature control	15-45oC (ambient +20/-7oC)

Note on working range: Values ka and kd are also dependent of each other, as well as the Bmax (binding maximum) values of the assay at the time, so in principle it is completely sample dependent range and the given ranges are just a guideline.

Extending working range: The range can be extended with careful experimental design. Please refer to J. Mol. Recognit. 2005; 18: 307–317 for more information.
Another option to extend the working range is Selectively Amplified Surface Plasmon Resonance (SAMP-SPR) technology that can be applied with SPR Navi™ instruments having additional wavelength (785 nm). SAMP-SPR is ideally suited to measure low concentrations of analyte in complex biological mixtures, with 100 times increased sensitivity and significantly reduced interference from background non-specific binding. This technology can be used to extend the range of affinity.

INSTRUMENT
Technique	Multi-Parametric Surface Plasmon Resonance (MP-SPR)
Measuring principle	True goniometric SPR with rotating laser (true angluar resolution 0.001o)
Wavelength of light	670 nm (and 785 nm with additional wavelength option) (other wavelengths available by request)
Optical channels	2
SPR angle scaning range	40o - 78o (with the same optics and same sensor slides and in the same scan, no need for prism exchange!, no manual manipulation needed)
Prism coating	Optical gel index matching (no need for messy oil operation between measurements)
Sensor slides	Au, Pt, Al, TiO2, SiO2, CMD, ... 12 x 20 mm
Refractive index (RI) range for the running buffer/bulk	1.00 - 1.45
Dynamic range	38 000 mdeg
Temperature of measurement chamber	15-45oC (ambient +20/-7oC) (in all models)
Temperature of sample storage	4-40°C at 25°C ambient temperature (only in 220A model!)
Computer requirements	The minimum PC requirements are: MS Windows® XP / VISTA / 7 (not Starter editions used in netbooks) both 32- and 64-bit versions are supported 1xUSB 2.0 2 GB RAM 2 Gb HD
Power requirements	100W, 100-240V, 50/60Hz

LIQUID HANDLING SPR NAVI™ 220A

	Liquid handling	Integrated Autosampler The instrument is equipped with two autosampling areas, one for small volumes and another for large volumes: Autosampling area 1: Fully configurable program Sample capacity: 1 microtiter plate (96 wells, both high and low format), 48 vials (1.5 mL, optional), or 12 vials (10 mL, optional) Injection modes: Full loop, partial loopfill Loop volume: 12 - 2000 μL programmable, exchangable, others by request Temperature controlled sample storage: 4-40°C Autosampling area 2: Fully configurable program Sample capacity: 6 samples in vials or bottles Injection modes: Full loop Wash: Programmable, wash between injections and wash between vials Loop volume: 12 - 2000 μL programmable, exchangable, others by request. Loops are standard liquid chromatography loops and can be exchanged easily.
		Integrated Syringe pump Sample syringe: 2 500 µL, 1-channel Measurement flow (running buffer) 2-channel 2500 μL standard Flowrates: 1µl/min to 6 mL/min
		Integrated Degasser 2-channel degasser Internal volume only 285 µl Eliminates baseline fluctuations, advanced error and leak checking functions Wetted parts: Instrument: FEP, PEEK, Systec AF, FFKM Flow-cell: PDMS/PEEK/titanium
	Dimensions	62 x 41 x 47 cm (24.5" x 16" x 18.5")
	Weight	30 kg (66 lbs)
	Shipping volumetric weight	50 kg (110 lbs)

LIQUID HANDLING SPR Navi™ 210A

	Liquid handling	Integrated Autosampler The instrument is equipped with one autosampling areas: Autosampling area: Fully configurable program Sample capacity: 6 samples in vials or bottles Injection modes: Full loop, partial loop Wash: Programmable, wash between injections and wash between vials Loop volume: 12 - 2000 μL programmable, exchangable, others by request. Loops are standard liquid chromatography loops and can be exchanged easily.
		Integrated Syringe pump Sample syringe: 2 500 µL, 1-channel Measurement flow (running buffer) 2-channel 2500 μL standard Flowrates: 1µl/min to 6 mL/min Integrated Degasser 2-channel degasser Internal volume only 285 µl Eliminates baseline fluctuations, advanced error and leak checking functions Wetted Parts Instrument: FEP (teflon-like), PEEK, Systec AF (teflon-like), FFKM Flow-cell: PDMS/PEEK/titanium

	DimensionsWeight	W 45 x H 41 x D 51 cm (18" x 16" x 20") 22 kg (49 lbs)
	Shipping volumetric weight	50 kg (110 lbs)

LIQUID HANDLING SPR NAVI™ 200

	Liquid handling	Pump: Integrated 2-channel peristaltic pump, software controlled, 10-400 µL/min
		Sample loading: Manual Loop volume: 100 µL (50 µL, 250 µL and others available) Injection: 12-port injector (manual switch) For experiments with liquids, we strongly recommend a degasser.The instrument can be equipped with a syringe pump or upgraded into SPR Navi 210A or 220A.Wetted parts: Instrument: PEEK Flow-cell: PDMS/PEEK/titanium
	Dimensions	W 32 x H 40 x D34 cm (15" x 12.5" x 12.4")
	Weight	12 kg (22 lbs)
	Shipping volumetric weight	50 kg (110 lbs)

Videos

https://bionavis.com/wp-content/uploads/2021/09/220a-naali-short-milk-concentration-22112018.mp4?_=1

Put yourself in the shoes of a scientist with our GoPro movie and check out how to measure α-lactalbumin protein concentration from bovine milk utilizing multi-parametric surface plasmon resonance!

Thanks to multiple wavelengths and scanning angular scans, BioNavis’ unique technology allows measurements of kinetic constants, concentration and competition analysis, affinity and EC50. MP-SPR provides insights into conformational changes of the molecules as well. PureKinetics™ is a unique feature of the MP-SPR Navi™ instruments that allows to measure the true binding of samples while the bulk signal can be reduced. Crude samples such as 100% serum, saliva or cell medium can be used.

Scientific Solutions

Scientific Solutions have been providing carefully selected laboratory and analytical equipment to Australian Industries such as Environmental, Pharmaceutical, Food and Beverage and Research markets of the Science Industry for over a decade.

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