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MP-SPR Navi™ 220A NAALI

Well plate automation for life sciences

MP-SPR Navi™ 220A NAALI measures not only interactions  providing premium quality data with  PureKinetics™, but also measures conformational changes and layer properties.

Product Category: SPR

Industry: Life Sciences, Particle and surface science

Supplier: Bionavis

Description

Features and benefits

  • Automation of samples from 96 and 384 well plates allows for unattended runs
  • Software switch for serial and parallel injections
  • Partial loop injections providing minimal sample consumption
  • Simultaneous  measurements  in  2  fluidic  channels
  • Two independent  wavelength  measurements  in each fluidic channel, enabling detection of thickness and refractive index or conformational changes
  • Instrument can be equipped optically with up to 4 different wavelenghts measured from each liquid channel for optimal data for layer characterization
  • Exceptionally  wide  angular  range 40˚ – 78˚ enables  measurements  of small  organic  molecules and  large  proteins,  but  also  metallic nanoparticles and cells. Most importantly, it enables in-line bulk effect separation with PureKinetics™
  • Measurements of kinetic constants, concentration and competition analysis, affinity and EC50
  • Controlled buffer flow conditions with precise syringe pumps and integrated degasser
  • Software package for convenient control of the instrument and easy analysis of the results. The instrument comes with TraceDrawer™ for  MP-SPR  Navi™  – a powerful  software  for  the determination  of affinity and  kinetic constants and more. MP-SPR Navi™ LayerSolver for the determination of thickness and RI from multiple wavelength measurements is an optional module
  • MP-SPR Navi™ instruments utilize optical RI matching elastomer and therefore, sensors are very easy to exchange and remove for further analysis with other methods such as AFM or microscopy
  • Complement with electrochemistry, high chemical resistant and gas flow cells as well as fluorescence setup provides combined or validation measurements

Specifications

SPR NAVI™ SPECIFICATIONS

ANALYTICAL
PERFORMANCE
Measured property SPR Navi™ instruments measure light intensity reflected from the sensor surface (absolute measurement), while traditional SPR instruments measure light shift (only a relative measurement).
Refractive index (RI) range 1.00 - 1.40 (Can be extended using additional wavelength option)
Using the same optics (prism and sensor slides) and in the same automated scan.
Dynamic range 38 000 mdeg
Noise level 0.3 µRIU (short term)
1 µRIU corresponds to 1 pg/mm² surface coverage of a typical protein.
100-fold improvement using Selectively Amplified Surface Plasmon Resonance.
Working range kon = 10E3 < ka < 10E8 M-1 s-1
koff = 0.1 < kd < 10E-7 s-1
KD = 10E-3 - 10E-12 M-1
Fastest sampling rate from 4 ms
in Fixed Angle advanced timing mode
Temperature control 15-45oC  (ambient +20/-7oC)

Note on working range: Values ka and kd are also dependent of each other, as well as the Bmax (binding maximum) values of the assay at the time, so in principle it is completely sample dependent range and the given ranges are just a guideline.

Extending working range: The range can be extended with careful experimental design. Please refer to J. Mol. Recognit. 2005; 18: 307–317 for more information.
Another option to extend the working range is Selectively Amplified Surface Plasmon Resonance (SAMP-SPR) technology that can be applied with SPR Navi™ instruments having additional wavelength (785 nm). SAMP-SPR is ideally suited to measure low concentrations of analyte in complex biological mixtures, with 100 times increased sensitivity and significantly reduced interference from background non-specific binding. This technology can be used to extend the range of affinity.

INSTRUMENT
Technique Multi-Parametric Surface Plasmon Resonance (MP-SPR)
Measuring principle True goniometric SPR with rotating laser (true angluar resolution 0.001o)
Wavelength of light 670 nm (and 785 nm with additional wavelength option)
(other wavelengths available by request)
Optical channels 2
SPR angle scaning range 40o - 78o
(with the same optics and same sensor slides and in the same scan, no need for prism exchange!, no manual manipulation needed)
Prism coating Optical gel index matching
(no need for messy oil operation between measurements)
Sensor slides  Au, Pt, Al, TiO2, SiO2, CMD, ...
12 x 20 mm
Refractive index (RI) range for the running buffer/bulk 1.00 - 1.45
Dynamic range 38 000 mdeg
Temperature of measurement chamber 15-45oC  (ambient +20/-7oC) (in all models)
Temperature of sample storage 4-40°C at 25°C ambient temperature (only in 220A model!)
Computer requirements The minimum PC requirements are:
MS Windows® XP / VISTA / 7 (not Starter editions used in netbooks)
both 32- and 64-bit versions are supported
1xUSB 2.0
2 GB RAM
2 Gb HD
Power requirements 100W, 100-240V, 50/60Hz

LIQUID HANDLING SPR NAVI™ 220A

Liquid handling Integrated Autosampler The instrument is equipped with two autosampling areas, one for small volumes and another for large volumes:

  • Autosampling area 1:
    • Fully configurable program
    • Sample capacity: 1 microtiter plate (96 wells, both high and low format), 48 vials (1.5 mL, optional), or 12 vials (10 mL, optional)
    • Injection modes: Full loop, partial loopfill
    • Loop volume: 12 - 2000 μL programmable, exchangable, others by request
    • Temperature controlled sample storage: 4-40°C
  • Autosampling area 2:
    • Fully configurable program
    • Sample capacity: 6 samples in vials or bottles
    • Injection modes: Full loop
    • Wash: Programmable, wash between injections and wash between vials
    • Loop volume: 12 - 2000 μL programmable, exchangable, others by request. Loops are standard liquid chromatography loops and can be exchanged easily.
Integrated Syringe pump

  • Sample syringe: 2 500 µL, 1-channel
  • Measurement flow (running buffer)
  • 2-channel
  • 2500 μL standard
  • Flowrates: 1µl/min to 6 mL/min
Integrated Degasser

  • 2-channel degasser
  • Internal volume only 285 µl
  • Eliminates baseline fluctuations, advanced error and leak checking functions

Wetted parts:

  • Instrument: FEP, PEEK, Systec AF, FFKM
  • Flow-cell: PDMS/PEEK/titanium
Dimensions  62 x 41 x 47 cm (24.5" x 16" x 18.5")
Weight  30 kg (66 lbs)
Shipping volumetric weight 50 kg (110 lbs)

LIQUID HANDLING SPR Navi™ 210A

Liquid handling Integrated Autosampler The instrument is equipped with one autosampling areas:

  • Autosampling area:
    •  Fully configurable program
    • Sample capacity: 6 samples in vials or bottles
    • Injection modes: Full loop, partial loop
    • Wash: Programmable, wash between injections and wash between vials
    • Loop volume: 12 - 2000 μL programmable, exchangable, others by request. Loops are standard liquid chromatography loops and can be exchanged easily.
Integrated Syringe pump

  • Sample syringe: 2 500 µL, 1-channel
  • Measurement flow (running buffer)
  • 2-channel
  • 2500 μL standard
  • Flowrates: 1µl/min to 6 mL/min

Integrated Degasser

  • 2-channel degasser
  • Internal volume only 285 µl
  • Eliminates baseline fluctuations, advanced error and leak checking functions

Wetted Parts

  • Instrument: FEP (teflon-like), PEEK, Systec AF (teflon-like), FFKM
  • Flow-cell: PDMS/PEEK/titanium
DimensionsWeight W 45 x H 41 x D 51 cm (18" x 16" x 20") 22 kg (49 lbs)
Shipping volumetric weight 50 kg (110 lbs)

LIQUID HANDLING SPR NAVI™ 200

Liquid handling Pump: Integrated 2-channel peristaltic pump, software controlled, 10-400 µL/min
Sample loading: Manual
Loop volume: 100 µL (50 µL, 250 µL and others available)
Injection: 12-port injector (manual switch)
For experiments with liquids, we strongly recommend a degasser.The instrument can be equipped with a syringe pump or upgraded into SPR Navi 210A or 220A.Wetted parts:

  • Instrument: PEEK
  • Flow-cell: PDMS/PEEK/titanium
Dimensions W 32 x H 40 x D34 cm  (15" x 12.5" x 12.4")
Weight 12 kg (22 lbs)
Shipping volumetric weight 50 kg (110 lbs)

 

Videos

https://bionavis.com/wp-content/uploads/2021/09/220a-naali-short-milk-concentration-22112018.mp4?_=1

 

Put yourself in the shoes of a scientist with our GoPro movie and check out how to measure α-lactalbumin protein concentration from bovine milk utilizing multi-parametric surface plasmon resonance!

Thanks to multiple wavelengths and scanning angular scans, BioNavis’ unique technology allows measurements of kinetic constants, concentration and competition analysis, affinity and EC50. MP-SPR provides insights into conformational changes of the molecules as well. PureKinetics™ is a unique feature of the MP-SPR Navi™ instruments that allows to measure the true binding of samples while the bulk signal can be reduced. Crude samples such as 100% serum, saliva or cell medium can be used.

Scientific Solutions

Scientific Solutions have been providing carefully selected laboratory and analytical equipment to Australian Industries such as Environmental, Pharmaceutical, Food and Beverage and Research markets of the Science Industry for over a decade.

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